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Preparation of oxidase reagent.
| Tetramethyl para-phenylene diamine dihydrochloride | 1.0 gm |
| Distilled water | 100 ml |
This reagent was made by dissolving 1 g of the reagent in 100 ml of distilled water. To that solution, stripes of Whatman No. 1 filter paper were soaked and drained for about 30 sec. Then, these stripes were completely dried and stored in a dark bottle tightly sealed with a screw cap.
