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Ingredients used in gram/liter in the preparation of nutrient agar.
| Ingredients | Gram/liter |
|---|---|
| Peptone | 5.0 |
| Sodium chloride | 5.0 |
| Beef extract | 1.5 |
| Yeast extract | 1.5 |
| Agar | 15.0 |
| PH (at 25°C) | 7.4 ± 0.2 |
About 28 gm of the medium was dissolved in 1000 ml of distilled water and heated to dissolve the media. The media were autoclaved at 15 lbs at 121°C for 15 minutes.
