Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Amount (in gm/ltr) of ingredients used in the preparation of nutrient broth.
| Ingredients | Gram/liter |
|---|---|
| Peptone | 5.0 |
| Sodium chloride | 5.0 |
| Beef extract | 1.5 |
| Yeast extract | 1.5 |
| PH (at 25°C) | 7.4 ± 0.2 |
About 13°gm of the medium was dissolved in 1000 ml of distilled water and heated to dissolve the media. The media were autoclaved at 15°lbs at 121°C for 15 minutes.
