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Ingredients in gram/liter used in the preparation of Mueller–Hinton agar (MHA).
| Ingredients | Gram/liter |
|
| |
| Beef extract | 2.0 |
| Casein acid hydrolysate | 17.5 |
| Starch | 1.5 |
| Agar | 17.0 |
| PH (at 25°C) | 7.4 ± 0.2 |
About 38 grams of the medium was suspended in 1000 ml water and boiled to dissolve completely. The media were then autoclaved at 15 lbs at 121°C for 15 minutes.
