Table 9.
Ingredients | Gram/liter |
| |
Tryptose | 20.0 |
Deoxyribonucleic acid | 2.0 |
Sodium chloride | 5.0 |
Methyl green | 0.0509 |
Agar | 15.0 |
PH (at 25°C) | 7.3 ± 0.2 |
About 42.05 grams of the medium was dissolved in 1000 ml distilled and boiled to dissolve completely. The media were then autoclaved at 15 lbs at 121°C for 15 minutes.