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hCPCs morphology and cardiac gene expression under normoxic and hypoxic microenvironments. Human CPCs were cultured under 21, 5, and 1% O2 for 48 h. DIC imaging shows the typical morphology of cells, which is unchanged under hypoxia. Immunofluorescent staining for cardiac lineage markers NKX-2.5 (green, nuclear), GATA-4 (green, nuclear), and c-kit (green) showed their expression was maintained under all oxygen conditions. Nuclei are stained blue and F-actin is stained red.
