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. 2020 Mar 6;8:130. doi: 10.3389/fcell.2020.00130

FIGURE 3.

FIGURE 3

Culturing hCPCs under hypoxia modulates hypoxic gene expression. hCPCs were cultured under 21, 5, and 1% O2 for 48 h then total RNA was harvested and gene expression analyzed via real-time PCR. Gene expression was normalized to five housekeeping genes and calculated as a fold change relative to levels at 21% O2. (A) 5% O2 culturing of hCPCs significantly increased expression of five genes, and significantly decreased expression of four genes. Data represented as mean ± SD, n = 3, all have p < 0.05 as compared to 21% O2. (B) 1% O2 culture altered expression of numerous hypoxia-related genes. 23 genes were significantly increased in expression and 8 genes were significantly decreased in expression, as compared to 21% O2. Data represented as mean ± SD, n = 3, all have p < 0.05 as compared to 21% O2. (C) Venn diagram illustrating similarly and differentially regulated genes under 5 and 1% O2, as compared to 21% O2.