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. 2020 Mar 6;8:130. doi: 10.3389/fcell.2020.00130

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Copyright © 2020 Dougherty, Patel, Kumar, Rao, Angelos, Singh, Cai and Khan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of hCPC-derived EVs on endothelial cell migration. BAECs were grown to confluence, wounded with a pipet tip, and treated with 100 μg/ml of total EV protein from each condition. (A) Representative images from 0 and 8 h show the extent of cell migration, with dotted lines approximating the cell front. (B) Wound healing assay demonstrates that treating ECs with hCPC-derived EVs increased cell migration significantly when compared to No-EVs group. However, no significant differences were observed between 5 and 1% O₂ derived EVs. Data are shown as mean ± SD, n = 4, *p < 0.05 vs. No-EVs control.