Figure 4.

HIF-1α Transcriptionally Suppresses HOXA9

(A) KB cells transfected with sh-NC or sh-HIF-1α. The protein levels of HIF-1α and HOXA9 were determined by western blotting. GAPDH served as a loading control. (B) Predicted binding site of HIF-1α in the promoter region of HOXA9 as determined by JASPAR. (C) EMSA of predicted binding motif. The DNA-protein complex is indicated by a black arrow, and the antibody supershift band is indicated by a white arrow. (D) The binding enrichment of HIF-1α on the promoter region of HOXA9 was detected by ChIP-PCR. Data are representative images or expressed as mean ± SD. ∗p < 0.05; ∗∗p < 0.01.