EphA2 Is the Target of 40L and A40s
(A) Reduction of EphA2 phosphorylation is shown through a receptor tyrosine kinase (RTK) proteome profile array after treating GSCs with 40L or G0. For control, reference spots of each array are shown. (B) A protein pull-down protocol is optimized for detection of A40s-binding proteins in GSC#1 and GSC#83. Eluted proteins were analyzed by mass spectrometry, and the most significant common membrane proteins are shown in the table. (C) EphA2 levels in several stem cells are compared to a differentiated counterpart. (D) The EphA2 level is shown in U251 derived stem-like cells, U87MG derived stem-like cells, and differentiated cells.