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. 2020 Feb 13;20:176–185. doi: 10.1016/j.omtn.2020.02.005

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© 2020 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

EphA2 Is the Target of 40L and A40s

(A) Reduction of EphA2 phosphorylation is shown through a receptor tyrosine kinase (RTK) proteome profile array after treating GSCs with 40L or G0. For control, reference spots of each array are shown. (B) A protein pull-down protocol is optimized for detection of A40s-binding proteins in GSC#1 and GSC#83. Eluted proteins were analyzed by mass spectrometry, and the most significant common membrane proteins are shown in the table. (C) EphA2 levels in several stem cells are compared to a differentiated counterpart. (D) The EphA2 level is shown in U251 derived stem-like cells, U87MG derived stem-like cells, and differentiated cells.