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TPX2 Is Confirmed as a Target Gene of miR-216b
(A) Luciferase activity of the TPX2-WT and TPX2-Mut after transfection determined by dual luciferase reporter gene assay. (B and C) Protein bands and protein levels of TPX2 in A431 cells normalized to GAPDH determined by western blot analysis. Data were expressed as mean ± standard deviation and analyzed by the unpaired t test. *p < 0.05.
