Figure 4.

Overexpressed miR-216b Inhibits Proliferation and Induces Apoptosis through Activation of the p53 Signaling by Downregulating TPX2 in cSCC

(A) miR-216b expression in A431 cells determined by qRT-PCR. (B) mRNA levels of p53, p21, TPX2, and MDM2 in A431 cells determined by qRT-PCR. (C) mRNA levels of proliferation marker PCNA and apoptosis-related factors bcl-2/bax in A431 cells determined by qRT-PCR. (D) Western blots of p53, p21, TPX2, MDM2, PCNA, and bcl-2/bax in A431 cells normalized to GAPDH. (E) Protein expressions of p53, p21, TPX2, MDM2 in A431 cells determined by western blot analysis. (F) Protein expressions of PCNA and bcl-2/bax in A431 cells determined by western blot analysis. Data were expressed as mean ± standard deviation, and data among multiple groups were compared by one-way ANOVA, followed by the Tukey’s post hoc test. *p < 0.05.