Figure 9. The effect of MLL5-sgRNA and E6-sgRNA alone or in combination with Cisplatin on the apoptosis of HeLa cells. Two days after transfection with MLL5-sgRNA, E6-sgRNA, or mock plasmids, the cells were treated with Cisplatin at its IC₅₀. Twenty-four hours later the total number of apoptotic cells was quantified, using Annexin V/‎‎7AAD flow cytometry method. Data is expressed as percentage of apoptotic cells in 10,000 events per sample. A: Untreated control cells, B: Cells transfected with PX458 Mock plasmid, C: Cells transfected with plasmids harboring MLL5-sgRNA, D: Cells transfected with plasmids harboring E6-sgRNA. In each plot, Q1, Q2, Q3, and Q4 are necrotic (Annexin V-/7AAD+), late apoptotic (Annexin V+/ 7AAD+), early apoptotic (Annexin V+ and 7-AAD-), and viable (Annexin V- and 7-‎AAD-) cells, respectively. On the graph, presented data are mean ± SD of total apoptotic cells. Data were analyzed using one-way ANOVA followed by LSD post-hoc test for multiple comparison. P > 0.05 was considered as statistical difference.