Table 1.
List of various lipopeptides encoding genes and their respective primer set for PCR amplification.
| S.No. | Lipopeptide Class | Gene | Primer Name | Primer Sequences (5′-3′) | Reference |
|---|---|---|---|---|---|
| 1. | Surfactins | ||||
| (a) | Surfactin | sfP | SFP-F1 SFP-R1 |
ATGAAGATTTACGGAATTTA TTATAAAAGCTCTTCGTACG |
[58] |
| (b) | Surfactin | Srfc | Sur3f Sur3r |
ACAGTATGGAGGCATGGTC TTCCGCCACTTTTTCAGTTT |
[57] |
| (c) | Surfactin | SrfA-A | srfAA-Fw srfAA-Rv |
AAAGGATCCAGCCGAAGGGTG TCATGGT AAAAAGCTTGTTTTTCTCAAAGAACCAGCG |
[59] |
| (d) | Surfactin | srfAA (Surfactin synthetase subunit 1) | SRFAF SRFAR |
TCGGGACAGGAAGACATCAT CCACTCAAACGGATAATCCTGA |
[60] |
| (e) | Surfactin | srfA | F3726 R3879 |
GAAGTCTTCAGCGGCGAACTG GGGTGGCTCCGTTTTTCTCG |
[56] |
| (f) | Surfactin | srfDB | SUR3F SUR3R |
ACAGTATGGAGGCATGGTC TTCCGCCACTTTTTCAGTTT |
[61] |
| 2. | Iturins | ||||
| (a) | Iturin A | ItuD | ItuD1f ItuD1r |
GATGCGATCTCCTTGGATGT ATCGTCATGTGCTGCTTGAG |
[57] |
| (b) | Iturin A | Itu-C | ituC-Fw ituC-Rv |
AAAGGATCCAAGCGTGCCTTTTACGGGAAA AAAAAGCTT AATGACGCCAGCTTTCTCTT |
[59] |
| (c) | Iturin | ituC (Iturin A synthetase C) | ITUCF ITUCR |
GGCTGCTGCAGATGCTTTAT TCGCAGATAATCGCAGTGAG |
[60] |
| 3. | Fengycins | ||||
| (a) | Fengycin | FenD | FenD1f FenD1d |
TTTGGCAGCAGGAGAAGTTT GCTGTCCGTTCTGCTTTTTC |
[62] |
| (b) | Fengycin | fenD (Fengycin synthetase) | FENDF FENDR |
GGCCCGTTCTCTAAATCCAT GTCATGCTGACGAGAGCAAA |
[60] |
| (c) | Fengycin | FenE | FenEF FenER |
GTTTCATGGCGGCGAGCACG GATTCGCGGGAAGCGGATTGAGC |
[62] |
| (d) | Fengycin | Fen | Af2-F Tf1-R |
GAATAYMTCGGMCGTMTKGA GCTTTWADKGAATSBCCGCC |
[63] |
| 4. | Bacillomycins | ||||
| (a) | Bacillomycin | bmyB (Bacillomycin L synthetase B) | BMYBF BMYBR |
GAATCCCGTTGTTCTCCAAA GCGGGTATTGAATGCTTGTT |
[60] |
| (b) | Bacillomycin D | BamC | Bacc1f Bacc1r |
GAAGGACACGGAGAGAGTC CGCTGATGACTGTTCATGCT |
[57] |
| (c) | Bacillomycin D | bam D | ITUD-F1 ITUD-R1 |
TTGAAYGTCAGYGCSCCTTT TGCGMAAATAATGGSGTCGT |
[64] |
| 5. | Bacilysin | ||||
| (a) | Bacilysin |
bacAB |
BACD-F1 BAMD-R1 |
AAAAACAGTATTGGTYATCGCTGA CCATGATGCCTTCKATRCTGAT |
[65] |
| (b) | Bacilysin |
bacAB |
BACAB-F1 BACAB-R1 |
CTTCTCCAAGGGGTGAACAG TGTAGGTTTCACCGGCTTTC |
|
| 6. | Ericin | ||||
| Ericin | eriB |
eriBF
eriBR |
GAWKNACWCCWTWTGG CCRCCATATCSWTMTRYYTC |
[66] | |
| 7. | Mersacidin | ||||
| (a) | Mersacidin | mrsA | MRSA-F1 MRSA-R1 |
GGGTATATGCGGTATAAACTTATG GTTTCCCCAATGATTTACCCTC |
[67] |
| (b) | Mersacidin | mrsM | MRSM-F1 MRSM-R1 |
AAATGACCCGGCATATGTTC TGCTGACTAACTGGAATTGGAA |
|
| 8. | Mycosubtilin | ||||
| (a) | Mycosubtilin | fenF | ITUD-F1 ITUD-R1 |
TTGAAYGTCAGYGCSCCTTT TGCGMAAATAATGGSGTCGT |
[68] |
| (b) | Mycosubtilin | mycC | MYCC-F1 MYCC-R1 |
AATCAATTGGCACGAACCTT ATCGCCCGTTTTGTACATTC |
|
| 9. | Zwittermicins | ||||
| (a) | Zwittermicin A | Zwit | ZWITF2 ZWITR1 |
TTGGGAGAATATACAGCTCT GACCTTTTGAAATGGGCGTA |
[61] |
| 10. | Kurstakins | ||||
| (a) | Kurstakins | Kur | Aks-F Tks-R |
TCHACWGGRAATCCAAAGGG CCACCDKTCAAAKAARKWATC |
[69] |
Where H denotes A or C or T, W-A or T, R-A or G, D- A or G or T, K- G or T, Y-C or T.