Figure 3.

Site-specific mutations, disrupting the N-linked glycosylations in GP, result in the restoration of pNK degranulation. HEK293T cells were either left untransfected (A) or transfected with wild-type GP (B), GP glycan deletion mutants (C), or mock vector (D), and cocultured with pNK cells in the presence of 25 U/ml rhIL2 (Edri et al., 2018). Effector cells were sampled and assayed for natural killer cell degranulation marker CD107a. (E) Plot shows overlay of CD107a stains of pNK cells cultured with the different target cells. (F) Summary plot shows degranulation level of pNK cells cultured with different target cells. Results are from two representative experiments of four replications. Graphs representing mean ± SEM. ***p < 0.001, Ordinary one-way ANOVA multiple comparisons test.