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. 2020 Mar;190(3):630–641. doi: 10.1016/j.ajpath.2019.11.009

Figure 5.

Figure 5

Effect of erythropoietin receptor signaling on amacrine and ganglion cells. A: Representative images of an AP2α- and DAPI-stained cryosection and RBPMSα-stained flat mount from an uninjured WT mouse at 8 weeks of age used to count amacrine cells and ganglion cells, respectively. Representative AP2α-positive amacrine cells are noted with arrows. Anti-mouse secondary antibody also labels Ig in the retinal vasculature, and representative stained vessels are noted with asterisk. B: Quantification of amacrine cells per 100 μm in central and peripheral retina. C: Quantification of ganglion cells per field in central and peripheral retina. Data are expressed as means ± SEM (B and C). n ≥ 8 images from at least four mice for amacrine cell counts for each treatment group (B); n ≥ 12 images from at least three mice for ganglion cell counts for each treatment group (C). *P < 0.05, **P < 0.01, and ***P < 0.001. Scale bars = 100 μm (A).