Gaf1 Is Required for Longevity
(A) Chronological lifespan assays in WT and gaf1Δ cells grown in EMM2 in the absence or presence of 8 μM Torin1. Error bars represent SDs from 3 independent cell cultures, each measured 3 times per time point.
(B) AUC for lifespan assays of WT and gaf1Δ mutant cells without or with Torin1 treatment. Vertical bars: Torin1-mediated increase in average AUC values for WT (black) and gaf1Δ (red), with the p value reflecting the significantly larger lifespan increase in WT than in gaf1Δ cells.