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. 2020 Mar 11;6(3):e03504. doi: 10.1016/j.heliyon.2020.e03504

Figure 1.

Figure 1

EBNA1 dependent long-term gene expression in neurons. A. Plasmid maps of vectors, EB vector and EBNA lacking EB vector (delta EBNA). B. A typical fluorescent images of GFP-actin transfected neurons cultured for 23 days (23DIV) with EB or delta EBNA vectors (Upper: GFP, Lower: Hoechst staining, scale bar 100μm). Transfections were done at 0 DIV and neurons were fix at 23DIV and stained. C. The number of GFP signal positive neurons and the intensity of the GFP fluorescence were significantly higher in case of EB vector than delta EBNA vector (GFP signal positive neurons; EB vector: 11.90 ± 1.39, n = 15, delta EBNA: 2.20 ± 0.66, n = 15, p < 0.005, GFP signal intensity; EB vector: 1943.7 ± 105.6, n = 689, delta EBNA: 306.1 ± 23.8, n = 696, p < 0.005). The number of fields and cells for quantification are indicated above each bar. D. mRNA comparison, Quantitative PCR shows high number of mRNA in EB vector transfected cultured neurons that delta EBNA vector transfected one.