Figure 7.

SLRP Expression Domains Are Altered in Tbx5^lx/lx;Osr2Cre Mutants

Dorsal view of wild-type forelimbs (A, E, and I) and hindlimbs (B, F, and J), and mutant forelimbs (C, G, and K) and hindlimbs (D, H, and L) between E12 to E12.5 processed for Dcn (A–D), Lum (E–H), and Kera (I–L) whole mount in situ hybridization. Arrow and arrowhead indicates the ectopic domain of Dcn and absence of its expression in the posterior zeugopodal region in mutant, respectively (C). Arrows in (E) and (I) show the central zeugopodal domain where Lum and Kera are generally excluded or expressed at low levels and arrows in (G) and (K) show the ectopic expression of these SLRPs in the central domain.