TMOD1 shRNAs suppresses TMOD1 expression in HeLa and CaSki cells. Total RNA and whole cell lysate were extracted from stable HeLa and CaSki cells transfected with control shRNA, TMOD1 shRNA-1, TMOD1 shRNA-2 or hRNA-resistant TMOD1 with TMOD1 shRNA-1 and subjected to (A and C) reverse transcription-quantitative PCR and (B and D) western blot analysis, respectively. The data are presented as the mean ± standard deviation (n=3). **P<0.001 vs. control shRNA group; #P<0.001 vs. TMOD1 shRNA-1 group. TMOD1, tropomodulin-1; Con, control; sh, short hairpin.