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Primer sequences used for reverse transcription-quantitative PCR analysis.
| Gene | Forward sequence (5′-3′) | Reverse primer (5′-3′) |
|---|---|---|
| ASPM | GGCCCTAGACAACCCTAACGA | AGCTTGGTGTTTCAGAACATC |
| BUB1B | AAATGACCCTCTGGATGTTTGG | GCATAAACGCCCTAATTTAAGCC |
| SPDL1 | CGAGAGCTAGCTGAGCGAAT | CAGCCTCTTTGAGCCTGCAT |
| GAPDH | AAGCCTGCCGGTGACTAAC | GTTAAAAGCAGCCCTGGTGAC |
ASPM, abnormal spindle-like microcephaly-associated protein; BUB1B, mitotic checkpoint serine/threonine-protein kinase BUB1 β; SPDL1, protein spindly.
