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. 2020 Mar 6;8:100. doi: 10.3389/fcell.2020.00100

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Copyright © 2020 Laudani, La Cognata, Iemmolo, Bonaventura, Villaggio, Saccone, Barcellona, Cavallaro and Sinatra.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Distribution of NF-H (green signals) in DPSC-derived neuronal-like stem cells after 7 days of culture. In DPSC-derived neuronal-like stem cells grew on glass (A) NF-H is widely observed in central area; on ECM (B) more cells exhibit polymerized neurofilament along neurites. Nuclei were stained with DAPI (C–D) and a merge was made (E–F). Images were captured using lasers with wavelenght of 405 and 488 nm. Scale bar corresponds to 20 μm.