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. 2020 Mar 6;8:100. doi: 10.3389/fcell.2020.00100

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Copyright © 2020 Laudani, La Cognata, Iemmolo, Bonaventura, Villaggio, Saccone, Barcellona, Cavallaro and Sinatra.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Gene expression change during differentiation. Densitometric results of semi-quantitative RT-PCR analysis demonstrate a significant increase in early (PAX6 and VIM) and mature (MAP2, NF-L, and NF-M) neuronal markers. mRNA levels were normalized to the amount of β-actin mRNA and represented in a heatmap. The data are expressed as mean ± standard deviation (***P < 0.001 vs. Control as determined by two-way ANOVA followed by Tukey–Kramer post hoc test) from at least three independent experiments.