Figure 6. Functional Gab3 is required for controlling trophoblast giant cell invasion.

a) Representative H&E- or cytokeratin-7-stained images of gd12.5 placentas showing decidua basalis (DB) with spiral arteries (SpA), junctional zone (JZ) and labyrinth from WT, Gab3^R27C, Gab3^KO, NK cell-depleted (PK-136 treatment) WT, or Gab3^KO injected with WT NK cells at gd9.5 females. b) Spiral artery remodeling was assessed by measuring the vessel to lumen ratio of spiral arteries on mid sagittal sections of gd12.5 placentas stained with H&E (n=4 mice, each symbol represents an individual placenta, lines represent mean ± s.e.m.). c) Loss of Gab3 is associated with increased TGC invasion within the maternal spiral arteries. The average depth of cytokeratin-7+ cell layer in SpA walls were quantified on mid sagittal sections of gd12.5 placentas stained with cytokeratin-7 antibody and a hematoxylin counterstain (n=4 mice, each symbol represents an individual placenta, lines represent mean ± s.e.m.). Statistical analysis was done using a one-way ANOVA with Tukey post-test. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001