. Author manuscript; available in PMC: 2021 Mar 20.

Published in final edited form as: J Pharm Biomed Anal. 2019 Dec 27;181:113073. doi: 10.1016/j.jpba.2019.113073

Table 5.

Pre-processing blood stability of NLG207 nanoparticles at room temperature and at 4°C

Nominal (CPT Equivalents)	Immediately After Preparation	3 hours at Room Temperature		24 Hours at 4°C
-Total CPT -[Free CPT]^*	GM (ng/mL)	GM (ng/mL)	MC (%)	GM (ng/mL)	MC (%)
50 ng/mL [1.5 ng/mL]	49.64	51.64	4.0	53.23	7.2
50 ng/mL [1.5 ng/mL]	1.39	2.58	86.0	1.40	1.0
500 ng/mL [15 ng/mL]	554.4	579.7	4.6	595.3	7.4
500 ng/mL [15 ng/mL]	16.83	28.74	70.8	14.40	−14.4
5000 ng/mL [150 ng/mL]	5240	5549	4.7	5035	−3.9
5000 ng/mL [150 ng/mL]	152.5	300.0	96.7	139.4	−8.6

Abbreviations: GM, grand mean; MC, mean change from fresh.

- Based on the totality of data, approximately 3% [within 2.5 to 3.5%] of total CPT from NLG207 NDCs is present as free CPT under standard conditions. The nominal concentrations of free CPT listed are 3% of the nominal total CPT concentrations.