Fig. 2.

Two structural variant callers were implemented on the same linked-read data, GROC-SVs and Long Ranger. Validation of candidate inversions was performed via long-read alignment across breakpoints, or de novo reassembly of breakpoint regions. Thirty-two inversions (9 inversions, 21 microinversions) could be confirmed in both breakpoints; extensive sharing of inversions between subspecies as well as between Ae. aegypti and Ae. mascarensis is seen for inversions on chromosomes 2 and 3. Notably, chromosome 1, the homomorphic chromosome that is the site of the sex-determining locus, shows no shared inversions between subspecies outside of the hybrid zone of Rabai