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. 2020 Mar 6;11:255. doi: 10.3389/fpls.2020.00255

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Copyright © 2020 Lugassi, Kelly, Arad, Farkash, Yaniv, Yeselson, Schaffer, Raveh, Granot and Carmi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Sugar stimulates the closure of citrus fruit stomata. The stomatal response to sucrose in WT fruit rind was assayed with rind discs (taken from six fruits) that were immersed for 2.5 h in artificial xylem sap (Wilkinson and Davies, 1997), artificial xylem sap containing 200 mM sorbitol (as an osmotic control), 100 mM sucrose (Suc), and 200 mM Suc. Epidermal imprints were then taken and the apertures of 60 stomata from each treatment were measured. Data are displayed as means ± SE. Asterisks denote significant differences relative to the WT (t-test, P < 0.01).