Table 4.

Primer sequences and expected amplicon sizes of the pathogenic Strains of E. coli (Pathotyping)

Target strain	Gene Target	Primer Sequence (5′–3′)	Amplicon Size (bp)
EAEC	Eagg	AGA CTC TGG CGA AAG ACT GTA TC	194
		ATG GCT GTC TGT AAT AGA TGA GAA C
EPEC	EaeA	CTG AAC GGC GAT TAC GCG AA	917
		GAC GAT ACG ATC CAG
ETEC	LT	GGC GAC AGA TTA TAC CGT GC	450
		CGG TCT CTA TAT TCC CTG TT
	ST	ATTTTTCTTTCTGTATTGTCTT	190
		CACCCGGTACAAGCAGGATT
EIEC	shig	CTGGTAGGTATGGTGAGG	320
		CCAGGCCAACAATTATTTCC