Table 2.
Precision analysis of the developed GSP270-LFIA test strip in testing HBsAg-positive serum with concentrations of 10, 50, 100, 200, and 500 ng/mL.
| Diluted  concentration (ng/mL)  | 
Intra-assay precisiona) | Inter-assay precisionb) | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Recovered concentration  | 
CV (%) | Recovery  (%)  | 
Recovered concentration | CV (%)  | 
Recovery  (%)  | 
|||||
| 500 | 491.8±9.89 | 2.01 | 98.36 | 502.89±65.32 | 12.99 | 100.58 | ||||
| 200 | 162.83±10.52 | 6.46 | 81.42 | 159.05±16.17 | 10.16 | 79.53 | ||||
| 100 | 105.08±8.27 | 7.87 | 105.08 | 110.17±6.98 | 6.34 | 110.17 | ||||
| 50 | 43.27±4.72 | 10.91 | 86.54 | 48.89±3.24 | 6.62 | 97.78 | ||||
| 10 | 9.02±1.03 | 11.41 | 90.20 | 9.17±1.23 | 13.41 | 91.70 | ||||
a) The assay was carried out in triplicates on the same day; b) The assay was performed on three consecutive days.