Figure 8.

The m⁶A/ ERRγ axis and in vivo cancer progression. (A) IHC (ERRγ and Mettl3)-stained paraffin-embedded sections obtained from HepG2 and HepG2/ADR xenografts when the tumor volumes were about 100 mm³ for each group; (B) The sh-control and sh-Mettl3 HepG2/ADR cells were subcutaneously inoculated in nude mice. IHC (P-gp and Cpt1b)-stained paraffin-embedded sections obtained at the end of experiment; (C) Tumor volume measurement in mouse xenografts. HepG2/ADR cells stably transfected with sh-ERRγ were subcutaneously inoculated in nude mice. We randomly divided the mice into sh-ERRγ, sh-ERRγ + Elacridar, sh-ERRγ + Etomoxir, and sh-ERRγ + Elacridar + Etomoxir and then treated with Dox as described in the Methods. Tumor growth curves were constructed based on the tumor volumes measured in the mice; (D) Expression of ESRRG in HCC tumor tissues and normal liver tissues from Oncomine database (Guichard and TCGA liver cancers); (E) ESRRG expression in liver cancers of T1 (n=153), T2 (n=77), and T3 (n=65) stages from TCGA database; (F) Expression of Mettl3 in HCC tumor tissues and normal liver tissues from Oncomine database (Roessler liver); (G) Correlation between ESRRG and ABCB1 in liver cancer patients (n=371) from TCGA database; Data were presented as means ± SD from three independent experiments. *p<0.05, **p< 0.01.