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. 2020 Feb 18;10(8):3562–3578. doi: 10.7150/thno.39944

Figure 3.

Figure 3

Repression of ENT1 expression and uptake of substrate entecavir under hypoxia. Four RCC cell lines (786-O, 769-P, Caki-1, ACHN) were exposed to normoxia or hypoxia for 48 h. Total RNA was isolated to determine ENT1 mRNA levels by real-time PCR (A) and membrane protein was extracted to determine the protein expression of ENT1 (B). ATP2A2 was used as a loading control of membrane extracts. (C) Accumulation of entecavir, a substrates of ENT1, in 786-O and 769-P was detected by LC-MS after exposure under hypoxia for 48 h. The protein expression of ENT1 (D, F) and uptake of entecavir (E, G) were detected in 786-O and 769-P cell lines, exposed to indicated periods (24, 48 or 72 h) of hypoxia or indicated oxygen concentration (8%, 4% or 1%) for 48 h.