Figure 4.

CFUS improved the local and systemic anti-tumor immunity in B16F10 melanoma model. (A) Mice were challenged in the contralateral flank with 1x10⁵ B16F10 cells 2 weeks post inoculation of the primary tumor (n=4-5). (B) Tumor volumes at the treated tumor site showing significant regression for CFUS and CRT-NPs despite pressure imposed on the immune system by tumor re-challenge. (C) Number of mice that were tumor free at the distant untreated site. Data are shown as mean ± SEM, * p < 0.05, ** p < 0.01; One-way ANOVA followed by Fisher's LSD without multiple comparisons correction.