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. 2020 Feb 19;10(8):3668–3683. doi: 10.7150/thno.41365

Figure 3.

Figure 3

Suppressive effects of miR-17-5p and miR-20a-5p on HCC metastasis in vitro and xenograft models. (A&C) Representative motility images. Bars, 200 μm. (B&D) Motility of HCCLM3 and HepG2 cells with indicated treatment. n=3. WT, NC, miR-17-5p-o, miR-20a-5p-o, or miR-17-5p-i, miR-20a-5p-i were defined as none, irrelevant oligonucleotides, miR-17-5p mimics, miR-20a-5p mimics, miR-17-5p inhibitors and miR-20a-5p inhibitors transfected cells, respectively. Student's t-test. ***p<0.001. (E) miR-17-5p/miR-20a-5p overexpressed HCCLM3 cells and miR-17-5p/miR-20a-5p knockdown HepG2 cells was in situ injected in Balb/c nude mice. The table shows the pulmonary metastasis and survival rate among different xenograft mice group by micro-spiral CT scan after 6 weeks. (F&H) Representative CT scanning images of metastatic nodules in lungs in indicated xenograft mice. The first panel: Coronal scanning images; The second panel: Cross-sectional scanning images; The third panel: Three-dimensional images. Red, normal lung tissue; Green, metastatic foci. The forth panel: Representative images of hematoxylin eosin (H&E) staining of metastatic nodules in lungs from indicated xenograft mice. (G) Tumor volumes of lung metastatic foci in indicated HepG2 xenografts. (I) The numbers of lung Metastatic foci in indicated HCCLM3 xenografts. N=5. Student's t-test. ***p<0.001. Data depicted as the mean ± SD from three independent experiments.