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. 2020 Feb 19;10(8):3708–3721. doi: 10.7150/thno.41677

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PRTC is able to interact with CREPT. (A) Molecular stimulation of the PRTC binding CREPT CCT domain using Schrodinger (Maestro 11.8) and SWISS-MODEL. (B) Microscale thermophoresis result of FITC-labeled PRTC with GST-tagged CREPT. Kd = 0.34 ± 0.11 μM. (PRTC, blue dot; PRTC-m, green dot). (C) Thermal shift assay results of GST-tagged CREPT with deionized H₂O (Ctrl), PRTC or PRTC-m. PRTC induced 6 °C shift in T_m compared to the control group. (D) Exogenous IP experiment of GST-tagged CREPT Ligand (CL) with Myc-CREPT and Myc-CREPT-CCT. (E) Exogenous IP experiment of Flag-CREPT with GST-CL or GST-CL-m in HEK293T cells. (F) GST pull-down experiments of Flag-CREPT with GST-tagged CL or CL-m using purified proteins from HEK293T cells.