Table 3.

Adenine cleavage rate and specificity^a

MutY	T (°C)	k2 (min−1) OG:A	Fold reduction	k2 (min−1) G:A	Fold reduction	Specificity
Ec MutY	37	12 (±2) b		1.80 (±0.05) b		7 (±1)
EcN MutY (1-224)	37	0.39 (±0.07) c		0.15 (±0.01) c		2.6 (±0.5)
Gs MutY	60	54 (±4)d		0.44 (±0.04) e		120 (±10)
EcNGsC	37	1.6 (±0.1)		0.33 (±0.02)		4.8 (±0.4)
	60	8 (±1)	1	1.0 (±0.1)	1	8 (±1)
FAH EcNGsC	60	5.1 (±0.2)	1.6 (±0.2)	0.6 (±0.1)	1.7 (±0.3)	8 (±1)
AAH EcNGsC	60	1.0 (±0.2)	8 (±2)	0.4 (±0.1)	2.5 (±0.7)	2.5 (±0.8)
Del FSH EcNGsC	60	0.6 (±0.1)	13 (±3)	0.5 (±0.1)	2.0 (±0.4)	1.2 (±0.3)

^aRate constant k₂ was measured using 20 nM DNA and excess enzyme in reaction mixtures containing 0.05 M sodium chloride. The k₂ values are reported as the average of at least three trials. Uncertainties are one standard deviation. Specificity was calculated as the ratio of k₂ rate constants measured for OG:A and G:A DNAs. Uncertainties for fold reduction and specificity were derived by propagation of error.

^bk₂ values previously reported.⁴⁰

^ck₂ values previously reported.¹⁵

^dk₂ values previously reported.²⁵

^ek₂ values previously reported.⁴¹