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. Author manuscript; available in PMC: 2020 Mar 13.
Published in final edited form as: Cell Rep. 2020 Jan 28;30(4):1235–1245.e4. doi: 10.1016/j.celrep.2019.12.067

Figure 1. Schematic Illustration of the ARK Assay.

Figure 1.

Cells are lysed with 5.6 M GTC to disrupt the noncovalent association between DNA and proteins. Free DNA and DPC-associated DNA are recovered by ethanol precipitation. The DNA-DPC pellet is dissolved in SDS buffer to further eliminate noncovalent DNA-protein interactions and to denature the proteins. The subsequent addition of KCl results in the precipitation of SDS-bound proteins along with DPC-associated DNA, while free DNA remains soluble. The DPCs that are recovered are digested with proteinase K to remove the protein adducts, resulting in the release of DPC-associated DNA for the quantification and calculation of the DPC coefficient.