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. Author manuscript; available in PMC: 2020 Mar 13.
Published in final edited form as: Cell Rep. 2020 Jan 28;30(4):1235–1245.e4. doi: 10.1016/j.celrep.2019.12.067

Figure 7. Removal of DPCs in Fanconi Anemia and NER Mutants.

Figure 7.

(A) Clonogenic survival of HeLa wild-type, XPA−/−, FANCL−/−, XPA−/−/FANCL−/− cells treated with FA.

(B) Removal of DPCs in wild-type HeLa cells and other indicated knockout derivatives. Cells were exposed to 500 μM FA for 2 h. DPC coefficients were determined for each cell line at indicated time points and normalized against the 0 time point to arrive at the percentages of DPC remaining.

(C) Left panel: DPC accumulation in wild-type HeLa cells and other indicated knockout derivatives continuously exposed to low-dose FA (75 μM) for 6 and 12 h; right panel: analysis of FA-induced DPC accumulation among HeLa cells examined in the left panel after formaldehyde treatment for 6 and 12 h, respectively. Accumulated DPCs are calculated from the DPC coefficient at the selected time point with a deduction of background level (time 0) for corresponding HeLa cell lines.

Number of biological repeats with duplication: n = 2 for (A), n = 4 for (B) and (C). The error bars depict standard deviation. One-way ANOVA analyses for the indicated time points (9 and 18 hr in B; 6 and 12 h in C) generated p < 0.0002 for the F-test, and interested pain/vise Tukey test results are displayed.