KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| anti-Tdp2 antibody | Bethyl Laboratories | Cat# A302-737A; RRID:AB_10631698 |
| anti-TOP1 antibody | Bethyl Laboratories | Cat# A302-589A; RRID:AB_2034865 |
| anti-dsDNA antibody | Abcam | Cat# ab27156; RRID:AB_470907 |
| anti-XPA antibody | Lab Vision | Cat# MS-650-P0; RRID:AB_142101 |
| anti-vinculin antibody | Sigma-Aldrich | Cat# V9131; RRID:AB_477629 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Formaldehyde (FA) | Sigma-Aldrich | Cat# F8775 |
| Guanidine thiocyanate (GTC) | Sigma-Aldrich | Cat# G9277 |
| Camptothecin (CPT) | Sigma-Aldrich | Cat# 390238 |
| PicoGreen | Thermo Fisher Scientific | Cat# P7589 |
| MycoAlert | Lonza | Cat# LT07-218 |
| Etoposide (ETO) | Abcam | Cat# ab120227 |
| Critical Commercial Assays | ||
| GeneArt Seamless Cloning and Assembly Kit | Thermo Fisher Scientific | Cat# A13288 |
| Neon Transfection Kit | Thermo Fisher Scientific | Cat# MPK10025 |
| Experimental Models: Cell Lines | ||
| 293A | Invitrogen | Cat# R70507 |
| TK6 | Dr. Shunichi Takeda | N/A |
| TK6 SPRTN−/− | This paper | N/A |
| TK6 TDP1−/− | Hoa et al., 2016 | N/A |
| TK6 TDP1/2−/− | This paper | N/A |
| HeLa | ATCC | ATCC CCL-2; RRID:CVCL_0030 |
| HeLa XPA−/− | This paper | N/A |
| HeLa FANCL−/− | Tian et al., 2017 | N/A |
| HeLa XPA−/−;FANCL−/− | This paper | N/A |
| Oligonucleotides | ||
| SPRTN gRNA: TCAAAAGGGGTTCGCTGAGACGG | This paper | N/A |
| TDP2 gRNA: CCAAGAAGGTCCAAACTTCG | This paper | N/A |
| XPA gRNA: GGCGGCTTTAGAGCAACCCG | This paper | N/A |
| TK6 Primers F/R 1-8 see Table S1 | This paper | N/A |
| Recombinant DNA | ||
| DT-A-pA/loxP/PGK- NeoR-pA/loxP | Laboratory for Animal Resources and Genetic Engineering, Center for Developmental Biology, RIKEN Kobe, http://www2.clst.riken.jp/arg/cassette.html | Cassette #16 |
| DT-A-pA/loxP/PGK-PuroR-pA/loxP | Ninagawa et al., 2014 | N/A |
| pX330-U6-Chimeric_BB-CBh-hSpCas9 | Cong et al., 2013 | Addgene Cat# 42230; RRID:Addgene_42230 |
| lentiGuide-Puro | Sanjana et al., 2014 | Addgene Cat# 52963; RRID:Addgene_52963 |
| lentiCas9-Blast | Sanjana et al., 2014 | Addgene Cat# 52962; RRID:Addgene_52962 |
| Software and Algorithms | ||
| ImageJ | ImageJ | https://imagej.nih.gov/ij/; RRID:SCR_003070 |
| GraphPad Prism 8 | GraphPad | https://www.graphpad.com/scientific-software/prism/; RRID:SCR_002798 |