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. 2020 Mar 6;7:111. doi: 10.3389/fvets.2020.00111

Figure 1.

Figure 1

Confocal microscopy images of A. phagocytophilum (Ap) binding/entering HL-60 and ISE6 cells. Cell nuclei are stained blue with DAPI, bacteria green by immunofluorescence (primary antibody: dog anti-Ap serum; secondary antibody: goat anti-dog IgG-FITC). (A) Sample 1 (Ap incubated with HL-60 for 2 h; (B) Sample 2 (Ap incubated with HL-60 for 4 h); (C) Sample 3 (Ap incubated with ISE6 for 2 h; (D) Sample 4 (Ap incubated with ISE6 for 4 h); and (E) Sample 5 (HL-60-grown bacteria incubated with ISE6 cells for 2 h).