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. 2020 Mar 6;7:111. doi: 10.3389/fvets.2020.00111

Figure 2.

Figure 2

Transcript levels in Sample 1 and sample 3, illustrating differential p44 transcription in HL-60 and ISE6, and antisense transcripts. Transcription data (top four graphs) plotted over the annotated genome sequence (sections at the bottom; turquoise boxes denote coding regions of annotated genes, pink boxes denote p44 conserved sequences with yellow boxes denoting their center hypervariable region) of A. phagocytophilum (Ap) strain HGE1 using the Artemis genome browser. Black bars represent A. phagocytophilum (Ap) transcription in sample 1 (2 h with HL-60 cells), and lavender bars transcription signal in sample 3 (2 h with ISE6 cells) (Infected). Each bar corresponds to one 60-mer probe on the tiling array. The bottom (Uninfected) graphs depict lack of signal from uninfected controls (HL-60 green, ISE6 blue). Prominent antisense signals in sample 3 are indicated (arrows), especially at the junction of HGE1_05172 (putative ATP synthase FO, B subunit) and HGE1_05177 (ATP synthase subunit C), but also from the p44 paralogs (pink bars with yellow centers) and HGE1_05187. HGE1_05192 (p44-18) has strong signal corresponding to its hypervariable region (yellow) indicating that it is specifically transcribed in sample 1. Nucleotide positions in the genome are indicated by numbers above the gray line.