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. 2020 Mar 6;7:111. doi: 10.3389/fvets.2020.00111

Figure 4.

Figure 4

Transcript levels in Samples 6 and 8, illustrating antisense signals and transcription from an unannotated gene. Transcription data (top four graphs) plotted over the annotated genome sequence (sections at the bottom; turquoise boxes denote coding regions of annotated genes) of A. phagocytophilum (Ap) strain HGE1 using the Artemis genome browser. Blue and black bars (top two graphs) represent Ap transcription in sample 6 (Ap replicating in HL-60 cells for 24 h, blue bars), and in and sample 8 (Ap replicating in human granulocytes, PMN, for 24 h, black bars). Each bar corresponds to one 60-mer probe on the tiling array. The bottom (Uninfected) graphs depict lack of signal from uninfected controls (HL-60 green, PMN blue). Antisense signals from infected HL-60 cells and granulocytes are indicated (arrows). A strong transcription signal (pink box) is visible from an unannotated gene immediately downstream of dnaK. Nucleotide positions in the genome are indicated by numbers above the gray line.