Table 1.

Samples for RNA extraction.

Sample Nr./Replicatesa	Origin host cellb	Target host cellc	Controld	Sampling timee
1/1	Ap from HL-60	HL-60	Ap w/o host cells	2 h
2/3	Ap from HL-60	HL-60	Ap w/o host cells	4 h
3/1	Ap from ISE6	ISE6	Ap w/o host cells	2 h
4/3	Ap from ISE6	ISE6	Ap w/o host cells	4 h
5/1	Ap from HL-60	ISE6	Ap w/o host cells	2 h
6/4	Ap from HL-60	HL-60	Uninfected HL-60	24 h
7/3	Ap from ISE6	ISE6	Uninfected ISE6	48 h
8/3	Ap from HL-60	PMNf	–	24 h

^aSample number and number of biological replicates used.

^bHost cells from which A. phagocytophilum were purified.

^cHost cells to which purified A. phagocytophilum were exposed.

^dPurified A. phagocytophilum incubated in freshly-made cell culture medium alone.

^eTime post of addition of A. phagocytophilum to target host cells when samples were taken.

^fHuman granulocytes.