Table 2. Primer sets of KIM-1, lipocalin-2 and GAPDH.

Component	Volume/reaction
5× miScript HiSpec buffer or 5× miScript Hiflex buffer	4 μl
10× nucleics mix	2 μl
RNase-free water	Variable
miScript reverse transcriptase mix	2 μl
Template RNA (added in step 3)	Variable (see Table 1 for recommendations)
Total volume	20 μl