Table 3. PCR Protocols for KIM-1, lipocalin-2 and GAPDH.

	KIM-1	Lipocalin-2	GAPDH
Initial activation	95 °C for 10 minutes
Number of cycles	40	40	40
Denaturation	95 °C for 15 seconds	95 °C for 15 seconds	95 °C for 15 seconds
Annealing	55 °C for 15 seconds	55 °C for 15 seconds	55 °C for 15 seconds
Extension	60 °C for 15 seconds	60 °C for 15 seconds	60 °C for 15 seconds
Final extension	60 °C for 10 minutes
Melting analysis