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. 2020 Mar 9;11(2):269–285. doi: 10.14336/AD.2019.0524

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Copyright: © 2020 Sun et al.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 3. — Exogenous H₂S protected mitochondria by maintaining of mitochondrial dynamics. (A) The expression levels of the mitochondrial dynamics-related proteins, P-Drp1/Drp1, Fis1 and Mfn2, were measured in cardiac mitochondria by Western blotting. (B) The expression levels of the mitochondrial dynamics-related proteins, P-Drp1/Drp1, Fis1 and Mfn2, were examined in neonatal rat cardiomyocytes by Western blotting. (C) The mitochondrial morphology of neonatal rat cardiomyocytes was measured by MitoTracker green assay. (D) JC-1 assay was used to examine the mitochondrial membrane potential of neonatal rat cardiomyocytes. (E) The expression of Parkin, PINK1, Beclin1, Atg7, P62 and LC3II/I was detected in neonatal rat cardiomyocytes with Mito-Tempo and Midivi-1 treatment in by Western blotting. Values are presented as the mean ± S.D. from n = 5 replicates. *P<0.05, **P<0.01, ***P<0.001.