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. 2020 Mar 9;11(2):269–285. doi: 10.14336/AD.2019.0524

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Copyright: © 2020 Sun et al.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 6. — Exogenous H₂S upregulated mitophagy through the activation of the USP8 signaling pathway. (A) The ubiquitination level of cytosolic parkin was examined following USP8 siRNA treatment by immunoprecipitation. (B) The ubiquitination level of mitochondrial Mfn2 was examined following USP8 siRNA treatment by immunoprecipitation. (C) Western blotting analysis and quantification of mitochondrial and cytoplasmic parkin protein under USP8 siRNA treatment. Values are presented as the mean ± S.D. from n = 4 replicates. **P<0.01vs Control, ***P<0.001 vs Control. (D) The expression of parkin in cytoplasma under USP8 siRNA and MG132 treatment. (E) Western blotting analysis of mitochondrial LC3B protein under USP8 siRNA treatment. Values are presented as the mean ± S.D. from n = 4 replicates. *P<0.05, **P<0.01.