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. 2020 Mar 6;11:228. doi: 10.3389/fimmu.2020.00228

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Copyright © 2020 Erdei, Tóth, Nagy, Nyakundi, Fejes, Nagy, Novák, Bognár, Balogh, Paragh, Kappelmayer, Bácsi and Jeney.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Pro-inflammatory effects of different Hb forms in HBECs. (A–D) Confluent HBECs (P5-8) were exposed to vehicle, Hb, metHb (MHb), ferrylHb (FHb), heme (25 μmol/L heme group), and LPS (100 ng/ml) in 1% FBS. Relative mRNA expression (4 h, mean ± SD) of VCAM-1, ICAM-1, and IL-8 normalized to GAPDH from three independent experiments performed in triplicates are shown. (B) VCAM-1 protein levels (16 h) were analyzed by western blot from whole cell lysate. Membranes were re-probed for β-actin. Representative blots of three independent experiments are shown. Densitometry analysis (mean ± SD) of three independent experiments. (A–D) P-values were calculated using one-way ANOVA followed by Dunnett’s post hoc analysis. *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001.