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. 2020 Mar 6;13:25. doi: 10.3389/fnmol.2020.00025

Figure 2.

Figure 2

Effects of TOCP on proliferation, differentiation, and cell apoptosis of mouse NSCs. NSCs were treated with TOCP (40 and 100 μM, respectively). (A) The cell proliferation was determined by EdU assay. Scale bar, 50 μm. (B) The neural differentiation-related proteins Tuj-1 (a neuronal marker) and glial fibrillary acidic protein (GFAP; an astrocyte marker) were detected by Western blot. (C) The cell apoptosis of TOCP-treated NSCs was assessed using annexin V–FITC/PI double staining by flow cytometry. (D) The apoptosis-related protein, cleaved caspase-3 was examined by Western blot. Data are presented as mean ± SD of at least three independent experiments. *P < 0.05, **P < 0.01 vs. control.