Figure 5.

Melatonin activates ERK1/2 signaling pathway suppressed by TOCP in mouse NSCs. NSCs were treated with 100 μM TOCP in the presence or absence of 50 μM PD98059 (a specific ERK1/2 inhibitor) and 40 μM melatonin, respectively. (A) The expression of p-ERK in TOCP-treated NSCs was examined by Western blot. (B) Autophagy-related protein, LC3, was analyzed by Western blot. Data are expressed as mean ± SD of at least three independent experiments. **P < 0.01 vs. control, ^#P < 0.05, ^##P < 0.01 vs. 100 μM TOCP alone.