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. 2020 Mar 9;15:1569–1583. doi: 10.2147/IJN.S220057

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© 2020 Ou et al.

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GO-PEI-inhibitor inhibits cell migration and metastasis in Cal27 cells. (A) Cal27 cell invasion was measured using Matrigel-coated chamber filters for 24 h. (B) Statistical analysis of the invading cell ratio is presented for each group. *P<0.05. **P<0.01. (C) The wounds of confluent Cal27 cells were created using 100 μL yellow tips and were incubated with lipo-inhibitor or GO-PEI-inhibitor (5 μg/mL). Images of the wounds at 0, 24 and 48 h are shown, and a cell-free gap is bordered by the pair of black lines. (D) Statistical analysis of cell migration was performed by calculating the ratio of the final cell-free gap to the initial wound area. *P<0.05. (E) The expression levels of snail1 and E-cadherin after treatment with lipo-inhibitor or GO-PEI-inhibitor were evaluated by qRT-PCR. *P<0.05.